[Problems to be solved] The object of the invention is to provide an aqueous textile printing composition having superior printing characteristics that can be used with multicolor rotating screen printing machines without causing environmental problems. [Means for solving the problems] (1) An aqueous tack-prevention textile printing composition for multicolor rotating screen printing machines, wherein one or more resins from the group consisting of saturated polyester resin, polyamide resin, polyethylene resin, polyurethane resin, ethylene vinyl ester copolymer resin, silicone resin, and acrylic silicone copolymer resin, which are tack prevention components, are used as a binder resin, (2) a continuous printing method, wherein after printing of an aqueous white-ground textile printing composition, printing of an aqueous tack-prevention textile printing composition is performed, then printing of an aqueous color textile printing composition is performed, wherein the textile printing composition according to (1) is used as the aqueous tack-prevention textile printing composition.