ENZYME DE COAGULATION DU LAIT PRODUITE PAR UNE BACTERIE ET PROCEDE CORRESPONDANT DE FABRICATION DE FROMAGE

N° de brevet: EP1535999 (A1)
Date de publication: 2005-06-01
Inventeur(s): YASOKAWA DAISUKE [JP];SAWADA HITOSHI [JP];NAKAGAWA RYOJI [JP];KAWAKAMI MAKOTO [JP];NAGASHIMA KOJI [JP];MIYASHITA SHUHEI [JP];MIYASHITA HIROKO [JP];
Demandeur(s): MAHOROBA CO LTD [JP];YASOKAWA DAISUKE [JP];
Classification: A23C19/032;C12N9/52;C12N9/54;C12P21/06;
N° de demande: EP20030738618 20030701 
Numéro(s) de priorité: WO2003JP08373 20030701;JP20020194016 20020702 
The present invention relates to a milk-coagulating enzyme of bacterial origin and a process for producing cheese by using this enzyme, namely, an enzyme that is produced by a bacterium belonging to the genus Paenibacillus and exhibits milk-coagulating activity, characterized by having the following enzymological properties: 1) function: having an activity of coagulating milk to form a curd; 2) substrate specificity: acting on kappa -casein as a substrate and specifically cleaving Thr94-Met95 in the presence of calcium; 3) optimum pH: 6.0-7.0, and 4) molecular weight: 35,000-37,000 Da when measured by SDS-PAGE; a process for producing the enzyme; a process for producing cheese; and a cheese-like food product. <IMAGE>

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